MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice

• Horie, Takahiro

• Nishino, Tomohiro

• Baba, Osamu

• Kuwabara, Yasuhide

• Nakao, Tetsushi

• Nishiga, Masataka

• Usami, Shunsuke

• Izuhara, Masayasu

• Sowa, Naoya

• Yahagi, Naoya

• Shimano, Hitoshi

• Matsumura, Shigenobu

• Inoue, Kazuo

• Marusawa, Hiroyuki

• Nakamura, Tomoyuki

• Hasegawa, Koji

• Kume, Noriaki

• Yokode, Masayuki

• Kita, Toru

• Kimura, Takeshi

• Ono, Koh

Abstract

MicroRNAs (miRs) are small non-protein-coding RNAs that bind to specific mRNAs and inhibit translation or promote mRNA degradation. Recent reports have indicated that miR-33, which is located within the intron of sterol regulatory element-binding protein (SREBP) 2, controls cholesterol homoeostasis and may be a potential therapeutic target for the treatment of atherosclerosis. Here we show that deletion of miR-33 results in marked worsening of high-fat diet-induced obesity and liver steatosis. Using miR-33−/−Srebf1+/− mice, we demonstrate that SREBP-1 is a target of miR-33 and that the mechanisms leading to obesity and liver steatosis in miR-33−/− mice involve enhanced expression of SREBP-1. These results elucidate a novel interaction between SREBP-1 and SREBP-2 mediated by miR-33 in vivo.

Journal

• Nature Communications

  Nature Communications 4 2013-12-03

  Nature Publishing Group

Keywords

• Biological sciences
• Medical research

Details

• CRID: 1050845760708122880
• NII Article ID: 120005349007
• ISSN: 20411723
• HANDLE: 2433/179546
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles