好熱性細菌 Thermus thermophilus 無機ピロリン酸加水分解酵素の error-prone PCR 変異導入による熱凝集抑制 Thermus thermophilus Inorganic Pyrophosphatase Variants by Error-prone PCR Suppressed Thermal Aggregation

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抄録

Thermus thermophilus Inorganic pyrophosphatase (E.C. 3.6.1.1., Tth PPase) exhibits high thermostability, but thermal aggregation was observed on heating above 85 °C. In addition, we reported that sole cysteine in C-terminal region plays a key role in the thermostability and thermal aggregation of Tth PPase [Kohaku, Y. et al. (2008) Natl.Sci.Res., 22, 75-84]. In this study, we approached the suppression of its thermal aggregation by error-prone PCR mutagenesis of whole molecule or C-terminal region. Firstly, we obtained thermostable four variants (Q119H/L162F, L162F, K173E and K159E/A170T) by error-prone PCR mutagenesis. Moreover, we examined thermostabilities of four variants in terms of the enzyme activity, tertiary and quaternary structure.Although conformation and quaternary structure of four variants were almost the same as those of wild type enzyme in native state, K173E and K159E/A170T variants showed higher thermostabilities than wild type in tertiary and quaternary structure. In particular, thermal aggregation of these two variants would be suppressed after heating at 85°C. Therefore, it was suggested that Lys159 and Lys173 in the molecular surface of C-terminal region may contribute to the formation of thermal aggregation of Tth PPase.

収録刊行物

  • 徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima

    徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima 27(4), 71-79, 2013-10

各種コード

  • NII論文ID(NAID)
    120005352782
  • NII書誌ID(NCID)
    AN10065859
  • 本文言語コード
    JPN
  • 資料種別
    Departmental Bulletin Paper
  • ISSN
    09146385
  • データ提供元
    IR 
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