Antibody-based analysis reveals "filamentous vs. non-filamentous" and "cytoplasmic vs. nuclear" crosstalk of cytoskeletal proteins.

• 粂田, 昌宏

• Hirai, Yuya

• Yoshimura, Shige H

• Horigome, Tsuneyoshi

• Takeyasu, Kunio

抄録

To uncover the molecular composition and dynamics of the functional scaffold for the nucleus, three fractions of biochemically-stable nuclear protein complexes were extracted and used as immunogens to produce a variety of monoclonal antibodies. Many helix-based cytoskeletal proteins were identified as antigens, suggesting their dynamic contribution to nuclear architecture and function. Interestingly, sets of antibodies distinguished distinct subcellular localization of a single isoform of certain cytoskeletal proteins; distinct molecular forms of keratin and actinin were found in the nucleus. Their nuclear shuttling properties were verified by the apparent nuclear accumulations under inhibition of CRM1-dependent nuclear export. Nuclear keratins do not take an obvious filamentous structure, as was revealed by non-filamentous cytoplasmic keratin-specific monoclonal antibody. These results suggest the distinct roles of the helix-based cytoskeletal proteins in the nucleus.

収録刊行物

• Experimental cell research

  Experimental cell research 319 (20), 3226-3237, 2013-12-10

  Elsevier Inc.

キーワード

• Cytoskeleton
• Keratin
• Monoclonal antibody
• Nuclear matrix
• Nuclear scaffold
• Nuclear transport

詳細情報

• CRID: 1050282810757245312
• NII論文ID: 120005353499
• NII書誌ID: AA00641011
• ISSN: 00144827
• HANDLE: 2433/179773
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles