マウスのタンパク質栄養状態と体毛タンパク質合成の関係について  [in Japanese] The Relationship between Protein Nutritional Status and Hair Protein Synthesis in Mice  [in Japanese]

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Abstract

タンパク質・エネルギー栄養失調症では,毛髪が細くなり,脆弱化することが知られている.しかし,毛髪の構成成分の変化については,ほとんど調べられていない.毛髪の主要な構成成分はタンパク質で,それは主にケラチンタンパク質(Keratin proteins:KP)とケラチン付随タンパク質(Keratin-associated proteins:KAP)に大別される.この2つのタイプのタンパク質は,分子量(KP>KAP)とシステイン含有率(KP<KAP)に違いがある.そこで,本研究では,毛髪を構成するタンパク質の違いに着目して,タンパク質欠乏食を与えたマウスの体毛タンパク質組成の変化について調べ,タンパク質栄養状態と体毛タンパク質合成の関係について検討を行った. 背部の体毛を除去した8週齢の雄C3Hマウスに25%カゼイン食(標準食群)あるいは1%カゼイン食(タンパク質欠乏食群)を4週間与えた.タンパク質欠乏食群では,体重が有意に減少し,また,血清総タンパク質は標準食群よりも有意に低値となった.体毛中システイン含有率は,標準食群と比較してタンパク質欠乏食群では有意に低値となった.また,体毛タンパク質の電気泳動よる分析から,タンパク質欠乏食群においてKAPの一部が減少していることが分かった. これらの結果は,タンパク質栄養状態が,システイン含有率の高いKAPの合成に影響することを示唆した.

It is known that hair becomes thin and weak during protein-energy malnutrition. However, the changes in hair components have not been well studied. The main component of hair is protein, of which there are 2 types: keratin proteins (KP) and keratin-associated proteins (KAP). These proteins differ in molecular weight (KP > KAP) and cysteine content rate (KP < KAP). In this study, we focused on the differences in hair proteins and examined changes in the composition of hair proteins in mice fed a protein-deficient diet. Eight-week-old male C3H mice, from which hair on the backs had been removed, were fed a diet containing 25% casein (standard diet group) or 1% casein (protein-deficient diet group) for 4 weeks. In the protein-deficient diet group, the body weight was significantly decreased and the total serum protein levels were significantly lower than those in the standard diet group. The cysteine content rate in the hair was significantly lower in the protein-deficient diet group compared to the standard diet group. Electrophoretic analysis of hair proteins indicated that there was a decrease in certain KAP in the protein-deficient group. These results suggest that protein nutritional status affects the synthesis of KAP with high cysteine content during hair formation.

Journal

  • 川崎医療福祉学会誌 = Kawasaki medical welfare journal

    川崎医療福祉学会誌 = Kawasaki medical welfare journal 22(2), 200-207, 2013

Codes

  • NII Article ID (NAID)
    120005358145
  • NII NACSIS-CAT ID (NCID)
    AN10375470
  • Text Lang
    JPN
  • Article Type
    departmental bulletin paper
  • Data Source
    IR 
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