The engraftment and differentiation of transplanted bone marrow-derived cells in the olfactory bulb after methimazole administration

• Noda, Yohei

• Nishizaki, Kazunori

• Yoshinobu, Junko

• Orita, Yorihisa

• Tsujigiwa, Hidetsugu

• Yamada, Masao

Abstract

Conclusion: Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb. Objectives: To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration. Methods: Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP. Results: GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.

Journal

• Acta Oto-laryngologica

  Acta Oto-laryngologica 133 (9), 951-956, 2013-09

Keywords

• Transplantation
• regeneration
• olfaction
• mitral cells
• microglia

Details

• CRID: 1050002212787616896
• NII Article ID: 120005359096
• ISSN: 00016489
• Web Site: https://ousar.lib.okayama-u.ac.jp/52019
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles