Relationship between neutrophil influx and oxidative stress in alveolar space in lipopolysaccharide-induced lung injury

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Abstract

We intratracheally administered lipopolysaccharide (LPS) to ICR mice and then collected BAL fluid and lung tissue to determine whether levels of neutrophils and/or myeloperoxidase (MPO) in bronchoalveolar lavage (BAL) fluid reflect lung tissue damage. Robust neutrophil accumulation into the alveolar space and lung tissue were almost completely abolished at seven days along with oxidative stress markers in the lung. However, lung injury scores and lung wet/dry ratios, as well as MPO and oxidative stress markers in BAL fluid were significantly increased at five and seven days after LPS administration. At later time points, BAL neutrophils generated more MPO activity and ROS than those harvested sooner after LPS administration. Although elevated neutrophil levels in BAL fluid reflected oxidative stress in the lungs, MPO might serve as a useful marker to evaluate damage sustained by epithelial cells over the long term. (C) 2013 Elsevier B.V. All rights reserved.

Journal

  • Respiratory physiology & neurobiology

    Respiratory physiology & neurobiology (191), 75-83, 2014-01-15

    Elsevier science bv

Codes

  • NII Article ID (NAID)
    120005411142
  • NII NACSIS-CAT ID (NCID)
    AA1161265X
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1569-9048
  • Data Source
    IR 
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