Generation of rat offspring derived from sperm cryopreserved/banked in the National BioResource Project for the rat followed by transportation to another institution

• Kashiwazaki, Naomi

• Seita, Yasunari

• Naoi, Kuniko

• Takizawa, Akiko

• Kuramoto, Takashi

• Ito, Junya

• Inomata, Tomo

• Serikawa, Tadao

抄録

To preserve genetic resources efficiently in rats, sperm cryopreservation is essential. This study aimed to confirm the ability of cryopreserved and transported rat spermatozoa to fertilize through intrauterine insemination. The Komeda miniature rat Ishikawa is a mutant caused by the autosomal recessive mutation mri. The epididymal sperm was frozen with egg yolk medium and banked at the National BioResource Project (NBRP), Kyoto University. The sperm was transported to Azabu University, then thawed at 37℃. The thawed semen was inseminated into the uterine horns of recipients; its motility was around 10%. Seven of 15 inseminated female rats became pregnant and 13 live pups were born. The results indicate that rat spermatozoa cryopreserved at NBRP are capable of restoring genetic resources through intrauterine insemination. We also confirmed the usefulness of assisted reproductive technologies for the rat including sperm cryopreservation and intrauterine insemination.

P(論文)

40016759566

原著論文

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ORIGINAL ARTICLE

収録刊行物

• 麻布大学雑誌

  麻布大学雑誌 17/18 31-34, 2009-03-31

  麻布大学

キーワード

• intrauterine insemination
• rat sperm
• sperm cryopreservation

詳細情報

• CRID: 1050001338029162240
• NII論文ID: 120005419216
• NII書誌ID: AA11561468
• ISSN: 13465880
• Web Site: https://az.repo.nii.ac.jp/records/3852
• Web Site: https://search.jamas.or.jp/link/ui/2009316313
• 本文言語コード: en
• 資料種別: departmental bulletin paper
• データソース種別:
  • IRDB
  • CiNii Articles