Generation of rat offspring derived from sperm cryopreserved/banked in the National BioResource Project for the rat followed by transportation to another institution

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Abstract

To preserve genetic resources efficiently in rats, sperm cryopreservation is essential. This study aimed to confirm the ability of cryopreserved and transported rat spermatozoa to fertilize through intrauterine insemination. The Komeda miniature rat Ishikawa is a mutant caused by the autosomal recessive mutation mri. The epididymal sperm was frozen with egg yolk medium and banked at the National BioResource Project (NBRP), Kyoto University. The sperm was transported to Azabu University, then thawed at 37℃. The thawed semen was inseminated into the uterine horns of recipients; its motility was around 10%. Seven of 15 inseminated female rats became pregnant and 13 live pups were born. The results indicate that rat spermatozoa cryopreserved at NBRP are capable of restoring genetic resources through intrauterine insemination. We also confirmed the usefulness of assisted reproductive technologies for the rat including sperm cryopreservation and intrauterine insemination.

Journal

  • 麻布大学雑誌 = Journal of Azabu University

    麻布大学雑誌 = Journal of Azabu University (17/18), 31-34, 2009-03-31

    Azabu University

Codes

  • NII Article ID (NAID)
    120005419216
  • NII NACSIS-CAT ID (NCID)
    AA11561468
  • Text Lang
    ENG
  • Article Type
    departmental bulletin paper
  • ISSN
    1346-5880
  • Data Source
    IR 
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