In vitro reconstitution of a CaMKII memory switch by an NMDA receptor-derived peptide.
抄録
Ca(2+)/Calmodulin-dependent protein kinase II (CaMKII) has been shown to play a major role in establishing memories through complex molecular interactions including phosphorylation of multiple synaptic targets. However, it is still controversial whether CaMKII itself serves as a molecular memory because of a lack of direct evidence. Here, we show that a single holoenzyme of CaMKII per se serves as an erasable molecular memory switch. We reconstituted Ca(2+)/Calmodulin-dependent CaMKII autophosphorylation in the presence of protein phosphatase 1 in vitro, and found that CaMKII phosphorylation shows a switch-like response with history dependence (hysteresis) only in the presence of an N-methyl-D-aspartate receptor-derived peptide. This hysteresis is Ca(2+) and protein phosphatase 1 concentration-dependent, indicating that the CaMKII memory switch is not simply caused by an N-methyl-D-aspartate receptor-derived peptide lock of CaMKII in an active conformation. Mutation of a phosphorylation site of the peptide shifted the Ca(2+) range of hysteresis. These functions may be crucial for induction and maintenance of long-term synaptic plasticity at hippocampal synapses.
収録刊行物
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- Biophysical journal
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Biophysical journal 106 (6), 1414-1420, 2014-03-18
Elsevier Inc.
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詳細情報 詳細情報について
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- CRID
- 1050282810768238720
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- NII論文ID
- 120005434969
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- ISSN
- 15420086
- 00063495
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- HANDLE
- 2433/187147
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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