Massive amounts of tissue factor induce fibrinogenolysis without tissue hypoperfusion in rats

• Hayakawa, Mineji

• Gando, Satoshi

• Ieko, Masahiro

• Honma, Yoshinori

• Homma, Taeko

• Yanagida, Yuichiro

• Kubota, Nobuhiko

• Uegaki, Shinji

• Sawamura, Atsushi

• Asakura, Hidesaku

抄録

Trauma-induced tissue factor (TF) release into the systemic circulation is considered to play an important role in the development of disseminated intravascular coagulation (DIC) immediately after severe trauma. However, the relationship between TF and hyperfibrinolysis, especially fibrinogenolysis, has been unclear. A total of 18 rats were divided into three groups: (a) the control group was infused with normal saline; (b) the low-dose group was infused with 4 U/kg TF; and (c) the high-dose group was infused with 16 U/kg TF. Arterial blood was drawn immediately and 2 and 4 h after the start of TF infusion. At each sampling point, arterial blood gases, platelet counts, and coagulation variables were measured. The fibrinogen degradation products were evaluated by a Western blot analysis. Hypotension, hypoxemia, and lactic acidosis were not observed in any of the three groups. In proportion to the doses of TF, the platelet counts, coagulation, and fibrinolysis variables deteriorated in line with DIC. The alpha 2-plasmin inhibitor levels significantly decreased in the high-dose group compared with the other groups. The amounts of fibrinogen degradation products increased in proportion to the doses of TF. The plasmin-alpha 2-plasmin inhibitor complex level in the high-dose group increased more than that of the other groups. In conclusion, TF can induce DIC associated with fibrinolysis and fibrinogenolysis without tissue hypoperfusion. The decrease in the alpha 2-plasmin inhibitor level and the significant increase in the plasmin level may be the two main factors underlying the pathogenesis of hyperfibrin(ogen)olysis after TF administration.

収録刊行物

• Shock

  Shock 39 (6), 514-519, 2013-06

  Lippincott Williams & Wilkins

キーワード

• Fibrin fibrinogen degradation products
• fibrinolysin
• fibrinolysis
• multiple trauma
• thromboplastin

詳細情報

• CRID: 1050845763944520064
• NII論文ID: 120005438221
• HANDLE: 2115/56243
• ISSN: 10732322
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles