DHX36 enhances RIG-I signaling by facilitating PKR-mediated antiviral stress granule formation.

• Yoo, Ji-Seung

• Takahasi, Kiyohiro

• Ng, Chen Seng

• Ouda, Ryota

• Onomoto, Koji

• Yoneyama, Mitsutoshi

• Lai, Janice Ching

• Lattmann, Simon

• Nagamine, Yoshikuni

• Matsui, Tadashi

• Iwabuchi, Kuniyoshi

• Kato, Hiroki

• 藤田, 尚志

抄録

RIG-I is a DExD/H-box RNA helicase and functions as a critical cytoplasmic sensor for RNA viruses to initiate antiviral interferon (IFN) responses. Here we demonstrate that another DExD/H-box RNA helicase DHX36 is a key molecule for RIG-I signaling by regulating double-stranded RNA (dsRNA)-dependent protein kinase (PKR) activation, which has been shown to be essential for the formation of antiviral stress granule (avSG). We found that DHX36 and PKR form a complex in a dsRNA-dependent manner. By forming this complex, DHX36 facilitates dsRNA binding and phosphorylation of PKR through its ATPase/helicase activity. Using DHX36 KO-inducible MEF cells, we demonstrated that DHX36 deficient cells showed defect in IFN production and higher susceptibility in RNA virus infection, indicating the physiological importance of this complex in host defense. In summary, we identify a novel function of DHX36 as a critical regulator of PKR-dependent avSG to facilitate viral RNA recognition by RIG-I-like receptor (RLR).

収録刊行物

• PLoS pathogens

  PLoS pathogens 10 (3), 2014-03-20

  Public Library of Science

詳細情報

• CRID: 1050001335791511040
• NII論文ID: 120005466661
• ISSN: 15537374
• HANDLE: 2433/187075
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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  • CiNii Articles
  • KAKEN