A chemical probe that labels human pluripotent stem cells.

• Hirata, Nao

• Nakagawa, Masato

• Fujibayashi, Yuto

• Yamauchi, Kaori

• Murata, Asako

• Minami, Itsunari

• Tomioka, Maiko

• Kondo, Takayuki

• Kuo, Ting-Fang

• Endo, Hiroshi

• Inoue, Haruhisa

• Sato, Shin-Ichi

• Ando, Shin

• Kawazoe, Yoshinori

• Aiba, Kazuhiro

• Nagata, Koh

• Kawase, Eihachiro

• Chang, Young-Tae

• Suemori, Hirofumi

• Eto, Koji

• Nakauchi, Hiromitsu

• Yamanaka, Shinya

• Nakatsuji, Norio

• Ueda, Kazumitsu

• Uesugi, Motonari

Abstract

A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.

Journal

• Cell reports

  Cell reports 6 (6), 1165-1174, 2014-03-27

  Elsevier B.V.

Details

• CRID: 1050001335791513088
• NII Article ID: 120005466668
• ISSN: 22111247
• HANDLE: 2433/187095
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles