Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel

• Ishida, Sumire

• Tanaka, Ryosuke

• Yamaguchi, Naoya

• Ogata, Genki

• Mizutani, Takeomi

• Kawabata, Kazushige

• Haga, Hisashi

• Alexandre J. Kabla

  editor

Abstract

Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-beta 1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-beta 1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement.

Journal

• PLOS ONE

  PLOS ONE 9 (8), e99655-, 2014-08-29

  Public Library of Science

Keywords

• Multidisciplinary

Details

• CRID: 1050001339016945920
• NII Article ID: 120005476150
• DOI: 10.1371/journal.pone.0099655
• HANDLE: 2115/57093
• ISSN: 19326203
• Web Site: http://dx.plos.org/10.1371/journal.pone.0099655
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN