Effect of substituting Trp for Leu at position 72 on the structure of Porphyromonas gingivalis superoxide dismutase

• 小町谷, 美帆

• 溝上, 真也

• 三原, 正志

• 大澤, 雅樹

• 菊池, 有一郎

• 上松, 節子

• 平井, 要

• 大久保, 裕一郎

• 黒岩, 昭弘

• 山田, 一尋

• 山倉, 文幸

• 平岡, 行博

• KOMACHIYA, MIHO

• MIZOUE, SHINYA

• MIHARA, MASASHI

• OSAWA, MASAKI

• KIKUCHI, YUICHIRO

• UEMATSU, SETSUKO

• OKUBO, YUICHIRO

• KUROIWA, AKIHIRO

• YAMADA, KAZUHIRO

• YAMAKURA, FUMIYUKI

• HIRAOKA, B.YUKIHIRO

Bibliographic Information

Other Title

• Porphyromonas gingivalis スーパーオキシドジスムターゼの構造における72位Leu をTrp に置換した影響

Abstract

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Porphyromonas gingivalis contains a single constitutive superoxide dismutase (SOD) that is active with either iron or manganese at the active site. The aim of this work was to evaluate the effect of the Leu ₇2 to Trp mutation on the structure of P. gingivalis SOD (Pg SOD) using lectrophoretic characterization. Leu ₇2, which is located near the active site metal, is substituted with Trp in aligned amino acid sequences of iron–containing SOD. The results of electrophoretic characterization and the expressed activity of mutant SOD suggest that mutant SOD have the same gross structure as wild–type SOD. We herein conclude that the integrity of Leu ₇2 is a necessary requisite for the metal–tolerant activity of Pg SOD.

Journal

• Journal of the Matsumoto Dental University Society

  Journal of the Matsumoto Dental University Society 40 (1), 19-25, 2014-06-30

  松本歯科大学学会

Keywords

• site–directed mutagenesis
• superoxide dismutase
• Porphyromonas gingivalis

Details

• CRID: 1050001338465857024
• NII Article ID: 120005487655
• ISSN: 03851613
• Web Site: https://mdu.repo.nii.ac.jp/records/2268
• Web Site: https://search.jamas.or.jp/link/ui/2015021013
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN