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Abstract

Artificial DNA cutters have been developed by us in our previous studies by combining two strands of pseudo-complementary peptide nucleic acid (pcPNA) with Ce(IV)–EDTA-promoted hydrolysis. The pcPNAs have two modified nucleobases (2,6-diaminopurine and 2-thiouracil) instead of conventional A and T, and can invade double-stranded DNA to activate the target site for the scission. This system has been applied to site-selective scissions of plasmid, λ-phage, E. coli genomic DNA, and human genomic DNA. Here, we have reported a still simpler and more convenient DNA cutter obtained by conjugating peptide nucleic acid (PNA) with a nuclear localization signal (NLS) peptide. This new DNA cutter requires only one PNA strand (instead of two) bearing conventional (non-pseudo-complementary) nucleobases. This PNA–NLS conjugate effectively activated the target site in double-stranded DNA and induced site-selective scission by Ce(IV)–EDTA. The complex formation between the conjugate and DNA was concretely evidenced by spectroscopic results based on time-resolved fluorescence. The target scission site of this new system was straightforwardly determined by the Watson–Crick base pairing rule, and mismatched sequences were clearly discriminated. Importantly, even highly GC-rich regions, which are difficult to be targeted by a previous strategy using pcPNA, were successfully targeted. All these features of the present DNA cutter make it promising for various future applications.

Journal

  • Organic & biomolecular chemistry

    Organic & biomolecular chemistry 11(32), 5233-5238, 2013-08

    The Royal Society of Chemistry

Keywords

Codes

  • NII Article ID (NAID)
    120005568080
  • NII NACSIS-CAT ID (NCID)
    AA1168650X
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1477-0520
  • Data Source
    IR 
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