Roles of Cytoplasmic Phospholipase in Expression of the Antimicrobial Activity of Host Macrophages against Mycobacterium tuberculosis Infection
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Abstract
We studied roles of phospholipase A2 (PLA2) isozymes, including type IIA secretory PLA2 (sPLA2-IIA), type IV cytosolic, Ca2+-dependent PLA2 (cPLA2) , type V secretory PLA2 (sPLA2-V) , and type VI cytosolic, Ca2+-independent PLA2 (iPLA2) , in macrophage (Mφ) antimicrobial activity against Mycobacterium tuberculosis (Mtb) H37Ra (avirulent) strain and Mφ mRNA expression of these PLA2 isotypes in response to infection with the microorganisms. First, a cPLA2 inhibitor arachidonyl trifluoromethylketone mildly reduced Mφ anti-Mtb activity, while the other PLA2 inhibitors did not significantly block the Mφ antimycobacterial function, if any. Second, Mφ expression of cPLA2 and sPLA2-V mRNAs was up-regulated during 6 to 12 h after infection with Mtb H37Ra strain. These findings suggest that cPLA2 plays a role in cellular mechanisms participating in the expression of Mφ anti-Mtb activity.
Journal
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- Shimane journal of medical science
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Shimane journal of medical science 27 17-24, 2010-12-01
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Details 詳細情報について
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- CRID
- 1050282814287019136
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- NII Article ID
- 120005586176
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- NII Book ID
- AA00841586
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- ISSN
- 03865959
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- Text Lang
- en
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- Article Type
- departmental bulletin paper
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- Data Source
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- IRDB
- CiNii Articles