Chronic treatment with a water-soluble extract from the culture medium of Ganoderma lucidum mycelia (MAK) prevents apoptosis and necroptosis in hypoxia/ischemia-induced injury of type 2 diabetic mouse brain
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Type 2 diabetes mellitus has been known to increase systemic oxidative stress by chronic hyperglycemia and visceral obesity and aggravate cerebral ischemic injury. On the basis of our previous study regarding a water-soluble extract from the culture medium of Ganoderma lucidum mycelia (designed as MAK), which exerts antioxidative and neuroprotective effects, the present study was conducted to evaluate the preventive effects of MAK on apoptosis and necroptosis, a programmed necrosis induced by hypoxia/ischemia (H/I) in type 2 diabetic KKAy mice. H/I was induced by a combination of unilateral common carotid artery ligation with hypoxia (8% O2 for 20 min) and subsequent reoxygenation. Pretreatment with MAK (1 g/kg, p.o.) for a week significantly reduced H/I-induced neurological deficits and brain infarction volume assessed at 24 h of reoxygenation. Histochemical analysis showed that MAK significantly suppressed superoxide production, neuronal cell death, and vacuolation in the ischemic penumbra, which was accompanied by a decrease in the numbers of TUNEL- or cleaved caspase-3-positive cells. Furthermore, MAK decreased the expression of receptor-interacting protein kinase 3 mRNA and protein, a key molecule for necroptosis. These results suggest that MAK confers resistance to apoptotic and necroptotic cell death and relieves H/I-induced cerebral ischemic injury in type 2 diabetic mice.
Open access journals
identifier:JOS-17414288_2015_865986
identifier:http://downloads.hindawi.com/journals/ecam/aip/865986.pdf
収録刊行物
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- Evidence-Based Complementary and Alternative Medicine
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Evidence-Based Complementary and Alternative Medicine 2015 (865986), 1-29, 2015-02-03
Hindawi Publishing Corporation
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詳細情報 詳細情報について
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- CRID
- 1050001202786752384
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- NII論文ID
- 120005590299
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- ISSN
- 17414288
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles