Macromolecular crowding effects on reactions of TePixD (Tll0078).

• Toyooka, Tsuguyoshi

• Tanaka, Keisuke

• Okajima, Koji

• Ikeuchi, Masahiko

• Tokutomi, Satoru

• Terazima, Masahide

Abstract

To reveal macromolecular crowding effects on a chemical reaction of a BLUF (sensors of blue light using FAD) protein (PixD from a thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 [TePixD, Tll0078]), the photoreaction was studied at various concentrations of the macromolecule Ficoll-70 by UV/Vis absorption spectroscopy and the pulsed laser-induced transient grating (TG) method. The absorption spectrum did not change with varying concentration of Ficoll-70. The crowding did not affect the quantum yield of the spectral red shift reaction, recovery rate of the product, rate constant of the volume change reaction and the magnitude of the volume change. However, the magnitude of the TG signal representing the diffusion-sensitive conformation change significantly increased on addition of Ficoll-70. This dependence was attributed to the crowding effect on the TePixD decamer-pentamer equilibrium in the solution. This result indicates that the TePixD reaction is more efficient in cellular than in in vitro conditions.

Journal

• Photochemistry and photobiology

  Photochemistry and photobiology 87 (3), 584-589, 2010-11-29

  wiley

Keywords

• Bacterial Proteins/chemistry
• Bacterial Proteins/genetics
• Bacterial Proteins/metabolism
• Cloning, Molecular
• Cyanobacteria/metabolism
• Diffusion/drug effects
• Diffusion/radiation effects
• Escherichia coli
• Ficoll/pharmacology
• Flavoproteins/chemistry
• Flavoproteins/genetics
• Flavoproteins/metabolism
• Light
• Molecular Conformation/drug effects
• Molecular Conformation/radiation effects
• Photochemical Processes/radiation effects
• Photoreceptors, Microbial/chemistry
• Photoreceptors, Microbial/genetics
• Photoreceptors, Microbial/metabolism
• Polymerization/radiation effects
• Protein Structure, Tertiary/drug effects
• Protein Structure, Tertiary/radiation effects
• Recombinant Proteins/chemistry
• Recombinant Proteins/genetics
• Recombinant Proteins/metabolism
• Spectrophotometry, Atomic

Details

• CRID: 1050564285764199552
• NII Article ID: 120005593641
• NII Book ID: AA00773103
• ISSN: 00318655
• HANDLE: 2433/197221
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles