A novel resting form of the trinuclear copper center in the double mutant of a multicopper oxidase, CueO, Cys500Ser/Glu506Ala

• Kajikawa, Takao

• Sugiyama, Ryosuke

• Kataoka, Kunishige

• Sakurai, Takeshi

Abstract

A multicopper oxidase, CueO was doubly mutated at its type I copper ligand, Cys500 and an acidic amino acid residue located in the proton transfer pathway, Glu506, to Ser and Ala, respectively. Cys500Ser/Glu506Ala was mainly in a novel resting form to afford the absorption band at ca. 400. nm and an EPR signal with a highly anisotropic character derived from type III copper. However, Cys500Ser/Glu506Ala gave the same reaction intermediate (peroxide intermediate) as that from Cys500Ser and Cys500Ser/Glu506Gln. © 2015 Elsevier Inc.

Embargo Period 24 months

Journal

• Journal of Inorganic Biochemistry

  Journal of Inorganic Biochemistry 149 88-90, 2015-08-01

  Elsevier

Keywords

• CueO
• Resting form
• Trinuclear copper center

Details

• CRID: 1050845760872609024
• NII Article ID: 120005613973
• NII Book ID: AA00258264
• ISSN: 01620134
• Web Site: http://hdl.handle.net/2297/42216
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles