Methylglyoxal Activates the Target of Rapamycin Complex 2-Protein Kinase C Signaling Pathway in <i>Saccharomyces cerevisiae</i>

• Nomura, Wataru

• 井上, 善晴

書誌事項

タイトル別名

• Methylglyoxal activates the target of rapamycin complex 2-protein kinase C signaling pathway in Saccharomyces cerevisiae.

抄録

Methylglyoxal is a typical 2-oxoaldehyde derived from glycolysis. We show here that methylglyoxal activates the Pkc1-Mpk1 mitogen-activated protein (MAP) kinase cascade in a target of rapamycin complex 2 (TORC2)-dependent manner in the budding yeast Saccharomyces cerevisiae. We demonstrate that TORC2 phosphorylates Pkc1 at Thr(1125) and Ser(1143). Methylglyoxal enhanced the phosphorylation of Pkc1 at Ser(1143), which transmitted the signal to the downstream Mpk1 MAP kinase cascade. We found that the phosphorylation status of Pkc1(T1125) affected the phosphorylation of Pkc1 at Ser(1143), in addition to its protein levels. Methylglyoxal activated mammalian TORC2 signaling, which, in turn, phosphorylated Akt at Ser(473). Our results suggest that methylglyoxal is a conserved initiator of TORC2 signaling among eukaryotes.

収録刊行物

• Molecular and cellular biology

  Molecular and cellular biology 35 (7), 1269-1280, 2015-04

  American Society for Microbiology

キーワード

• Enzyme Activation
• Mitogen-Activated Protein Kinases/metabolism
• Multiprotein Complexes/metabolism
• Phosphorylation
• Protein Kinase C/metabolism
• Pyruvaldehyde/metabolism
• Saccharomyces cerevisiae/cytology
• Saccharomyces cerevisiae/enzymology
• Saccharomyces cerevisiae/metabolism
• Saccharomyces cerevisiae Proteins/metabolism
• Signal Transduction
• TOR Serine-Threonine Kinases/metabolism

詳細情報

• CRID: 1050845760743568256
• NII論文ID: 120005615637
• ISSN: 10985549
• DOI: 10.1128/mcb.01118-14
• HANDLE: 2433/198443
• Web Site: https://journals.asm.org/doi/pdf/10.1128/MCB.01118-14
• Web Site: https://www.tandfonline.com/doi/pdf/10.1128/MCB.01118-14
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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