Downregulation of Pgc-1α expression by tea leaves and their by-products.
抄録
Previous studies indicate that muscle Pgc-1α expression governs the proportion of muscle fibre types. As a first step in using diet to manipulate the proportion of muscle fibre types by using Pgc-1α expression, the present study investigates the modulation of Pgc-1α expression by feedstuffs. A luciferase-based Pgc-1α reporter construct (Pgc-1α(-2553)-luc) that contains the mouse Pgc-1α promoter (-2553 to +78 bp) was prepared. A screen of ethanol extracts from 33 feedstuffs indicated that oolong tea and roasted green tea extracts decreased Pgc-1α(-2553)-luc expression in C2C12 myoblasts. The transcriptional repression of Pgc-1α by tea leaf extracts was reproduced in hepatic HepG2 cells. We further examined the effects of the alcohol extracts of tea waste and its silage on Pgc-1α transcription; the tea waste silage extract inhibited Pgc-1α transcription. Treatment with the extracts of raw tea leaves, tea waste and tea waste silage effectively decreased Pgc-1α mRNA levels during myogenesis of myosatellite cells. The present results suggest that tea leaves and their by-products could be used to modulate proportions of muscle fibre types.
収録刊行物
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- Cell biochemistry and function
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Cell biochemistry and function 32 (3), 236-240, 2013-09-20
wiley
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キーワード
- Pgc-1α
- skeletal muscle
- tea leaf
- muscle fibre type
- feedstuffs
- Animals
- Camellia sinensis/chemistry
- Cells, Cultured
- Down-Regulation
- Male
- Plant Extracts/pharmacology
- Plant Leaves/chemistry
- Promoter Regions, Genetic
- Rats
- Rats, Wistar
- Satellite Cells, Skeletal Muscle/drug effects
- Satellite Cells, Skeletal Muscle/metabolism
- Tea
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription, Genetic
詳細情報 詳細情報について
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- CRID
- 1050564285766925824
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- NII論文ID
- 120005623240
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- ISSN
- 02636484
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- HANDLE
- 2433/198734
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles
