TCDD-induced chick cardiotoxicity is abolished by a selective cyclooxygenase-2 (COX-2) inhibitor NS398

• Fujisawa, Nozomi

• Nakayama, Shouta M.M.

• Ikenaka, Yoshinori

• Ishizuka, Mayumi

Abstract

Halogenated aromatic hydrocarbons, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), are known to cause severe heart defects in avian species. However, the mechanism of TCDD-induced chick cardiovascular toxicity is unclear. In this study, we investigated cyclooxygenase-2 (COX-2) as a possible mechanism of TCDD-induced cardiotoxicity. Fertile chicken eggs were injected with TCDD and a COX-2 selective inhibitor, NS398, and we investigated chick heart failure on day 10. We found that the chick heart to body weight ratio and atrial natriuretic factor mRNA expression were increased, but this increase was abolished with treatment of NS398. In addition, the morphological abnormality of an enlarged ventricle resulting from TCDD exposure was also abolished with co-treatment of TCDD and NS398. Our results suggested that TCDD-induced chick heart defects are mediated via the nongenomic pathway and that they do not require the genomic pathway.

Journal

• Archives of Toxicology

  Archives of Toxicology 88 (9), 1739-1748, 2014-09

  Springer

Keywords

• Atrial natriuretic factor
• Cardiac malformation
• Chick embryo
• Cyclooxygenase-2 inhibitor
• Nongenomic pathway
• TCDD

Details

• CRID: 1050001202677775616
• NII Article ID: 120005649117
• NII Book ID: AA00548865
• HANDLE: 2115/59756
• ISSN: 03405761
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles