Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies

• Amengual, Olga

• Horita, Tetsuya

• Binder, Walter

• Norman, Gary L.

• Shums, Zakera

• Kato, Masaru

• Otomo, Kotaro

• Fujieda, Yuichiro

• Oku, Kenji

• Bohgaki, Toshiyuki

• Yasuda, Shinsuke

• Atsumi, Tatsuya

Abstract

Phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) were strongly correlated with the presence of lupus anticoagulant showing a high specificity for the diagnosis of antiphospholipid syndrome. However, the main criticism for the clinical applicability of aPS/PT testing is the lack of reproducibility of the results among laboratories. In this study, we measured IgG and IgM aPS/PT using our original in-house enzyme-linked immunosorbent assays (ELISA) and commercial ELISA kits to assess the assay performance and to evaluate the accuracy of aPS/PT results. The study included 111 plasma samples collected from patients and stored at our laboratory for aPS/PT assessment. Sixty-one samples were tested for IgG aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite (TM) aPS/PT IgG ELISA kit (INOVA Diagnostics, Inc., USA). Fifty samples were evaluated for IgM aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite (TM) aPS/PT IgM ELISA kit (INOVA Diagnostics). Ninety-eight percent of samples yielded concordant results for IgG aPS/PT and 82 % for IgM aPS/PT. There was an excellent agreement between the IgG aPS/PT assays (Cohen kappa = 0.962) and moderate agreement between the IgM aPS/PT assays (kappa = 0.597). Statistically significant correlations in the aPS/PT results were obtained from both IgG and IgM aPS/PT assays (r = 0.749, r = 0.622, p < 0.001, respectively). In conclusion, IgG and IgM detection by ELISA is accurate. The performance of aPS/PT is reliable, and concordant results can be obtained using different ELISA methods.

Journal

• Rheumatology international

  Rheumatology international 34 (9), 1225-1230, 2014-09

  Springer

Keywords

• Antiphospholipid syndrome
• Antiphospholipid antibodies
• Thrombosis
• Lupus anticoagulant

Details

• CRID: 1050001202927413504
• NII Article ID: 120005656328
• HANDLE: 2115/59853
• ISSN: 01728172
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles