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Abstract

We demonstrate the single-molecule imaging of the catalytic reaction of a Zn(2+) -dependent DNAzyme in a DNA origami nanostructure. The single-molecule catalytic activity of the DNAzyme was examined in the designed nanostructure, a DNA frame. The DNAzyme and a substrate strand attached to two supported dsDNA molecules were assembled in the DNA frame in two different configurations. The reaction was monitored by observing the configurational changes of the incorporated DNA strands in the DNA frame. This configurational changes were clearly observed in accordance with the progress of the reaction. The separation processes of the dsDNA molecules, as induced by the cleavage by the DNAzyme, were directly visualized by high-speed atomic force microscopy (AFM). This nanostructure-based AFM imaging technique is suitable for the monitoring of various chemical and biochemical catalytic reactions at the single-molecule level.

Journal

  • Angewandte Chemie: International Edition

    Angewandte Chemie: International Edition 54(36), 10550-10554, 2015-07-17

    wiley

Codes

  • NII Article ID (NAID)
    120005672611
  • NII NACSIS-CAT ID (NCID)
    AA0052535X
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1433-7851
  • Data Source
    IR 
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