Grease matrix as a versatile carrier of proteins for serial crystallography.

• Sugahara, Michihiro

• Mizohata, Eiichi

• Nango, Eriko

• Suzuki, Mamoru

• Tanaka, Tomoyuki

• Masuda, Tetsuya

• Tanaka, Rie

• Shimamura, Tatsuro

• Tanaka, Yoshiki

• Suno, Chiyo

• Ihara, Kentaro

• Pan, Dongqing

• Kakinouchi, Keisuke

• Sugiyama, Shigeru

• Murata, Michio

• Inoue, Tsuyoshi

• Tono, Kensuke

• Song, Changyong

• Park, Jaehyun

• Kameshima, Takashi

• Hatsui, Takaki

• Joti, Yasumasa

• Yabashi, Makina

• Iwata, So

Abstract

Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

Journal

• Nature methods

  Nature methods 12 (1), 61-63, 2014-11-10

  Nature Publishing Group

Keywords

• Nanocrystallography
• Proteins
• Aldose-Ketose Isomerases/chemistry
• Crystallization
• Crystallography, X-Ray/methods
• Fatty Acid-Binding Proteins/chemistry
• Lasers
• Lubricants
• Mineral Oil
• Muramidase/chemistry
• Plant Proteins/chemistry
• Proteins/chemistry

Details

• CRID: 1050564285772285952
• NII Article ID: 120005682565
• ISSN: 15487105
• HANDLE: 2433/203008
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles