Cell fate of müller cells during photoreceptor regeneration in an N-methyl-N-nitrosourea- induced retinal degeneration model of zebrafish

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Abstract

Zebrafish can regenerate several organs such as the tail fin, heart, central nervous system, and photoreceptors. Very recently, a study has demonstrated the photoreceptor regeneration in the alkylating agent N-methyl-N-nitrosourea (MNU)- induced retinal degeneration (RD) zebrafish model, in which whole photoreceptors are lost within a week after MNU treatment and then regenerated within a month. The research has also shown massive proliferation of Müller cells within a week. To address the question of whether proliferating Müller cells are the source of regenerating photoreceptors, which remains unknown in the MNU-induced zebrafish RD model, we employed a BrdU pulse-chase technique to label the proliferating cells within a week after MNU treatment. As a result of the BrdU pulse-chase technique, a number of BrdU+ cells were observed in the outer nuclear layer as well as the inner nuclear layer. This implies that regenerating photoreceptors are derived from proliferating Müller cells in the zebrafish MNU-induced RD model. © Springer International Publishing Switzerland 2016.

[Book Chapter]

Journal

  • Advances in Experimental Medicine and Biology

    Advances in Experimental Medicine and Biology (854), 685-692, 2016-10-01

    Springer

Codes

  • NII Article ID (NAID)
    120005682636
  • NII NACSIS-CAT ID (NCID)
    AA00512642
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0065-2598
  • Data Source
    IR 
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