5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability.

• Kafer, Georgia Rose

• Li, Xuan

• Horii, Takuro

• Suetake, Isao

• Tajima, Shoji

• Hatada, Izuho

• Carlton, Peter Mark

Abstract

5-hydroxymethylcytosine (5hmC) is a DNA base created during active DNA demethylation by the recently discovered TET enzymes. 5hmC has essential roles in gene expression and differentiation. Here, we demonstrate that 5hmC also localizes to sites of DNA damage and repair. 5hmC accumulates at damage foci induced by aphidicolin and microirradiation and colocalizes with major DNA damage response proteins 53BP1 and γH2AX, revealing 5hmC as an epigenetic marker of DNA damage. Deficiency for the TET enzymes eliminates damage-induced 5hmC accumulation and elicits chromosome segregation defects in response to replication stress. Our results indicate that the TET enzymes and 5hmC play essential roles in ensuring genome integrity.

Journal

• Cell reports

  Cell reports 14 (6), 1283-1292, 2016-02-16

  Elsevier Inc.

Keywords

• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1050845760757086336
• NII Article ID: 120005707793
• ISSN: 22111247
• DOI: 10.1016/j.celrep.2016.01.035
• HANDLE: 2433/207261
• Web Site: https://api.elsevier.com/content/article/PII:S2211124716000565?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S2211124716000565?httpAccept=text/plain
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN