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Abstract
Protein design is a useful method to create novel artificial proteins. A rational approach to design a heterodimeric protein using domain swapping for horse myoglobin (Mb) was developed. As confirmed by X-ray crystallographic analysis, a heterodimeric Mb with two different active sites was produced efficiently from two surface mutants of Mb, in which the charges of two amino acids involved in the dimer salt bridges were reversed in each mutant individually, with the active site of one mutant modified. This study shows that the method of constructing heterodimeric Mb with domain swapping is useful for designing artificial multiheme proteins.
Journal
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- Angewandte Chemie International Edition
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Angewandte Chemie International Edition 54 (2), 511-515, 2014-11-04
WILEY-VCH Verlag
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Details 詳細情報について
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- CRID
- 1050858784329444864
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- NII Article ID
- 120005716859
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- NII Book ID
- AA11614189
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- ISSN
- 15213773
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- HANDLE
- 10061/10411
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- CiNii Articles