<Originals> Expression of the Th1 surface marker, Tim-3, on allergen-stimulated T cells of allergen-sensitve donors
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Abstract
[Abstract] Background : The “Th2 hypothesis for asthma” remains controversial, because IFN-γ is not always suppressed in allergic asthma. Our objectives were therefore to identify the characteristics of IFN-γ-producing CD4^+ T cells that were induced by exposure to an allergen. Methods : Peripheral blood mononuclear cells (PBMC) were stimulated with house dust mites (HDM) and non-allergeic ubiquitous antigen M. tuberculosisPPD for 7 and 14 days, and then intracellular cytokines, IFN-γ and IL-4, and surface markers, Tim-3 and CRTH2, which were specifically identified in Th1 and Th2 cells, respectively, were quantified by flow cytometry. Results : We found that stimulation with HDM for 14 days generated IL-4- as well as IFN-γ-producing CD4^+ T cells (6.1±0.7% and 24.0±6.2%, respectively). In addition, stimulation with HDM induced Tim-3 expression on IFN-γ-producing CD4^+ T cells and CRTH2 expression on IL-4-producing CD4^+ T cells (16.1±7.5% vs. 0.9±0.5% and 21.2±6.7% vs. 9.6±4.7%, respectively, compared to no stimulation). On the other hand, very few IFN-γ- and IL-4-producing CD4^+ T cells stimulated with PPD expressedTim-3 and CRTH2. Conclusions : In vitro allergen-stimulated PBMCs contributed to the differentiation of Th2 as well as Th1 cells. A substantial fraction of Th1 cells expressed the expression of Tim-3, which is a death signal.
Departmental Bulletin Paper
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Journal
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- ACTA MEDICA KINKI UNIVERSITY
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ACTA MEDICA KINKI UNIVERSITY 36 (1), 1-6, 2011-06-01
The Kinki University Medical Association
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Details 詳細情報について
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- CRID
- 1050001202539552512
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- NII Article ID
- 120005736790
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- ISSN
- 03866092
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- Text Lang
- en
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- Article Type
- departmental bulletin paper
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- Data Source
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- IRDB
- CiNii Articles