Helical rearrangement of photoactivated rhodopsin in monomeric and dimeric forms probed by high-angle X-ray scattering

• 今元, 泰

• Kojima, Keiichi

• Oka, Toshihiko

• Maeda, Ryo

• 七田, 芳則

抄録

Light-induced helical rearrangement of vertebrate visual rhodopsin was directly monitored by high-angle X-ray scattering (HAXS), ranging from Q (= 4π sin θ/λ) = 0.03 Å-1 to Q = 1.5 Å-1. HAXS of nanodiscs containing a single rhodopsin molecule was performed before and after photoactivation of rhodopsin. The intensity difference curve obtained by HAXS agreed with that calculated from the crystal structure of dark state rhodopsin and metarhodopsin II, indicating that the conformational change of monomeric rhodopsin in the membrane is consistent with that occurring in the crystal. On the other hand, the HAXS intensity difference curve of nanodiscs containing two rhodopsin molecules was significantly reduced, similar to that calculated from the crystal structure of the deprotonated intermediate, without a large conformational change. These results suggest that rhodopsin is dimerized in the membrane and that the interaction between rhodopsin molecules modulates structural changes.

収録刊行物

• Photochemical and Photobiological Sciences

  Photochemical and Photobiological Sciences 14 1965-1973, 2015-08-05

  Royal Society of Chemistry

詳細情報

• CRID: 1050845760760050944
• NII論文ID: 120005741088
• ISSN: 14749092
• HANDLE: 2433/209998
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles