Decreased expression of a gene caused by a T-DNA insertion in an adjacent gene in arabidopsis

• Tamura, Kentaro

• Kawabayashi, Takenori

• Shikanai, Toshiharu

• 西村, いくこ

• Matsuo Uemura

  editor

抄録

ALADIN is a component of the nuclear pore complex in higher eukaryotes. An Arabidopsis knockout line that had a T-DNA insertion in the ALADIN gene was defective in plant growth and thylakoid development and had reduced photosynthetic activity resulting from lower chlorophyll accumulation. The mutation appeared to decrease the level of chloroplast RuBisCO subunits and PSBA and PGL35 proteins. Unexpectedly, the T-DNA insertion in the ALADIN gene decreased the expression of the neighboring gene PSRP5, which functions in translation in chloroplasts. The mutant phenotype was rescued by expressing PSRP5, but not by expressing ALADIN. The abnormal phenotypes were also detected in an artificial microRNA (amiRNA)-mediated PSRPS5 knockdown, but not in an amiRNA-mediated ALADIN knockdown line. Thus, users of T-DNA insertions should be aware that a TDNA insertion in one gene can have effects on the expression of neighboring genes.

収録刊行物

• PLOS ONE

  PLOS ONE 11 (2), e0147911-, 2016-02-01

  Public Library of Science

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050282810806696576
• NII論文ID: 120005743647
• ISSN: 19326203
• DOI: 10.1371/journal.pone.0147911
• HANDLE: 2433/210206
• Web Site: http://dx.plos.org/10.1371/journal.pone.0147911
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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