Critical roles for murine Reck in the regulation of vascular patterning and stabilization

• De Almeida, Glícia Maria

• Yamamoto, Mako

• Morioka, Yoko

• Ogawa, Shuichiro

• Matsuzaki, Tomoko

• Noda, Makoto

Abstract

Extracellular matrix (ECM) is known to play several important roles in vascular development, although the molecular mechanisms behind these remain largely unknown. RECK, a tumor suppressor downregulated in a wide variety of cancers, encodes a membrane-anchored matrix-metalloproteinase-regulator. Mice lacking functional Reck die in utero, demonstrating its importance for mammalian embryogenesis; however, the underlying causes of mid-gestation lethality remain unclear. Using Reck conditional knockout mice, we have now demonstrated that the lack of Reck in vascular mural cells is largely responsible for mid-gestation lethality. Experiments using cultured aortic explants further revealed that Reck is essential for at least two events in sprouting angiogenesis; (1) correct association of mural and endothelial tip cells to the microvessels and (2) maintenance of fibronectin matrix surrounding the vessels. These findings demonstrate the importance of appropriate cell-cell interactions and ECM maintenance for angiogenesis and the involvement of Reck as a critical regulator of these events.

A Corrigendum to this article was published on 04 March 2016. This article has been updated.

Journal

• Scientific Reports

  Scientific Reports 5 17860-, 2015-12-11

  Nature Publishing Group

Keywords

• Multidisciplinary

Details

• CRID: 1050001335835656064
• NII Article ID: 120005818244
• ISSN: 20452322
• DOI: 10.1038/srep17860
• HANDLE: 2433/215933
• Web Site: https://www.nature.com/articles/srep17860.pdf
• Web Site: https://www.nature.com/articles/srep17860
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN