Kaposi's sarcoma-associated herpesvirus-encoded LANA associates with glucocorticoid receptor and enhances its transcriptional activities

• Togi, Sumihito

• Nakasuji, Misa

• Muromoto, Ryuta

• Ikeda, Osamu

• Okabe, Kanako

• Kitai, Yuichi

• Kon, Shigeyuki

• Oritani, Kenji

• Matsuda, Tadashi

抄録

Kaposi's sarcoma-associated herpesvirus (KSHV)-encoded latency-associated nuclear antigen (LANA), which interacts with cellular proteins, plays a central role in modification of viral and/or cellular gene expression. Here, we show that LANA associates with glucocorticoid receptor (GR), and that LANA enhances the transcriptional activity of GR. Co-immunoprecipitation revealed a physical interaction between LANA and GR in transiently transfected 293T and HeLa cells. In human B-lymphoma cells, LANA overexpression enhanced GR activity and cell growth suppression following glucocorticoid stimulation. Furthermore, confocal microscopy showed that activated GR was bound to LANA and accumulated in the nucleus, leading to an increase in binding of activated GR to the glucocorticoid response element of target genes. Taken together, KSHV-derived LANA acts as a transcriptional co-activator of GR. Our results might suggest a careful use of glucocorticoids in the treatment of patients with KSHV-related malignancies such as Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman disease. (C) 2015 Elsevier Inc. All rights reserved.

収録刊行物

• Biochemical and biophysical research communications

  Biochemical and biophysical research communications 463 (3), 395-400, 2015-07-31

  Elsevier

キーワード

• KSHV
• LANA
• GR
• Cross-talk
• Transcription

詳細情報

• CRID: 1050845763948426368
• NII論文ID: 120005820702
• HANDLE: 2115/62600
• ISSN: 0006291X
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles