In vivo imaging of clock gene expression in multiple tissues of freely moving mice

• Hamada, Toshiyuki

• Sutherland, Kenneth

• Ishikawa, Masayori

• Miyamoto, Naoki

• Honma, Sato

• Shirato, Hiroki

• Honma, Ken-ichi

Abstract

Clock genes are expressed throughout the body, although how they oscillate in unrestrained animals is not known. Here, we show an in vivo imaging technique that enables long-term simultaneous imaging of multiple tissues. We use dual-focal 3D tracking and signal-intensity calibration to follow gene expression in a target area. We measure circadian rhythms of clock genes in the olfactory bulb, right and left ears and cortices, and the skin. In addition, the kinetic relationship between gene expression and physiological responses to experimental cues is monitored. Under stable conditions gene expression is in phase in all tissues. In response to a long-duration light pulse, the olfactory bulb shifts faster than other tissues. In Cry1(-/-) Cry2(-/-) arrhythmic mice circadian oscillation is absent in all tissues. Thus, our system successfully tracks circadian rhythms in clock genes in multiple tissues in unrestrained mice.

Journal

• Nature communications

  Nature communications 7 11705-, 2016-06-10

  Nature

Keywords

• General Physics and Astronomy
• General Biochemistry, Genetics and Molecular Biology
• General Chemistry
• Multidisciplinary

Details

• CRID: 1050001339020592256
• NII Article ID: 120005820742
• NII Book ID: AA12645905
• DOI: 10.1038/ncomms11705
• HANDLE: 2115/62597
• ISSN: 20411723
• Web Site: https://www.nature.com/articles/ncomms11705.pdf
• Web Site: https://www.nature.com/articles/ncomms11705
• Text Lang: en
• Article Type: journal article
• Data Source:
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  • Crossref
  • CiNii Articles
  • KAKEN