Selective autophagic receptor p62 regulates the abundance of transcriptional coregulator ARIP4 during nutrient starvation

• Tsuchiya, Megumi

• Isogai, Shin

• Taniguchi, Hiroaki

• Tochio, Hidehito

• Shirakawa, Masahiro

• Morohashi, Ken Ichirou

• Hiraoka, Yasushi

• Haraguchi, Tokuko

• Ogawa, Hidesato

Abstract

Transcriptional coregulators contribute to several processes involving nuclear receptor transcriptional regulation. The transcriptional coregulator androgen receptor-interacting protein 4 (ARIP4) interacts with nuclear receptors and regulates their transcriptional activity. In this study, we identified p62 as a major interacting protein partner for ARIP4 in the nucleus. Nuclear magnetic resonance analysis demonstrated that ARIP4 interacts directly with the ubiquitin-Associated (UBA) domain of p62. ARIP4 and ubiquitin both bind to similar amino acid residues within UBA domains; therefore, these proteins may possess a similar surface structure at their UBA-binding interfaces. We also found that p62 is required for the regulation of ARIP4 protein levels under nutrient starvation conditions. We propose that p62 is a novel binding partner for ARIP4, and that its binding regulates the cellular protein level of ARIP4 under conditions of metabolic stress.

Journal

• Scientific Reports

  Scientific Reports 5 14498-, 2015-09-28

  Nature Publishing Group

Keywords

• Multidisciplinary

Details

• CRID: 1050282810812408960
• NII Article ID: 120005820894
• ISSN: 20452322
• DOI: 10.1038/srep14498
• HANDLE: 2433/216105
• Web Site: https://www.nature.com/articles/srep14498
• Web Site: https://www.nature.com/articles/srep14498.pdf
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN