RPT2/NCH1 subfamily of NPH3-like proteins is essential for the chloroplast accumulation response in land plants

• Suetsugu, Noriyuki

• Takemiya, Atsushi

• Kong, Sam-Geun

• Higa, Takeshi

• Komatsu, Aino

• Shimazaki, Ken-ichiro

• Kohchi, Takayuki

• Wada, Masamitsu

Abstract

In green plants, the blue light receptor kinase phototropin mediates various photomovements and developmental responses, such as phototropism, chloroplast photorelocation movements (accumulation and avoidance), stomatal opening, and leaf flattening, which facilitate photosynthesis. In Arabidopsis, two phototropins (phot1 and phot2) redundantly mediate these responses. Two phototropin-interacting proteins, NONPHOTOTROPIC HYPOCOTYL 3 (NPH3) and ROOT PHOTOTROPISM 2 (RPT2), which belong to the NPH3/RPT2-like (NRL) family of BTB (broad complex, tramtrack, and bric à brac) domain proteins, mediate phototropism and leaf flattening. However, the roles of NRL proteins in chloroplast photorelocation movement remain to be determined. Here, we show that another phototropin-interacting NRL protein, NRL PROTEIN FOR CHLOROPLAST MOVEMENT 1 (NCH1), and RPT2 redundantly mediate the chloroplast accumulation response but not the avoidance response. NPH3, RPT2, and NCH1 are not involved in the chloroplast avoidance response or stomatal opening. In the liverwort Marchantia polymorpha, the NCH1 ortholog, MpNCH1, is essential for the chloroplast accumulation response but not the avoidance response, indicating that the regulation of the phototropin-mediated chloroplast accumulation response by RPT2/NCH1 is conserved in land plants. Thus, the NRL protein combination could determine the specificity of diverse phototropin-mediated responses.

Journal

• Proceedings of the National Academy of Sciences of the United States of America

  Proceedings of the National Academy of Sciences of the United States of America 113 (37), 10424-10429, 2016-08-30

  National Academy of Sciences

Keywords

• Arabidopsis
• blue light
• chloroplast movement
• Marchantia
• phototropin

Details

• CRID: 1050845760768326912
• NII Article ID: 120005829445
• ISSN: 10916490
• ISSN: 00278424
• DOI: 10.1073/pnas.1602151113
• HANDLE: 2433/216419
• Web Site: http://www.pnas.org/syndication/doi/10.1073/pnas.1602151113
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.1602151113
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN