Update on the Keio collection of Escherichia coli single‐gene deletion mutants

• Yamamoto, Natsuko

• Nakahigashi, Kenji

• Nakamichi, Tomoko

• Yoshino, Mihoko

• Takai, Yuki

• Touda, Yae

• Furubayashi, Akemi

• Kinjyo, Satoko

• Dose, Hitomi

• Hasegawa, Miki

• Datsenko, Kirill A

• Nakayashiki, Toru

• Tomita, Masaru

• Wanner, Barry L

• Mori, Hirotada

抄録

The Keio collection (Baba et al, 2006) has been established as a set of single‐gene deletion mutants of Escherichia coli K‐12. These mutants have a precisely designed deletion from the second codon from the seventh to the last codon of each predicted ORF. Further information is available at http://sal.cs.purdue.edu:8097/GB7/index.jsp or http://ecoli.naist.jp/. The distribution is now being handled by the National Institute of Genetics of Japan (http://www.shigen.nig.ac.jp/ecoli/pec/index.jsp). To date more than 4 million samples have been distributed worldwide. As we described earlier (Baba et al, 2006), gene amplification during construction is likely to have led to a small number of mutants with genetic duplications.

収録刊行物

• Molecular Systems Biology

  Molecular Systems Biology 5 (1), 2009-12-22

  Nature Publishing Group

詳細情報

• CRID: 1050295834376174080
• NII論文ID: 120005830849
• ISSN: 17444292
• HANDLE: 10061/10975
• Web Site: https://naist.repo.nii.ac.jp/records/9151
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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  • CiNii Articles