Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation

• Kimura, Shunsuke

• Yamashita, Masami

• Yamakami-Kimura, Megumi

• Sato, Yusuke

• Yamagata, Atsushi

• Kobashigawa, Yoshihiro

• Inagaki, Fuyuhiko

• Amada, Takako

• Hase, Koji

• Iwanaga, Toshihiko

• Ohno, Hiroshi

• Fukai, Shuya

Abstract

The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.

Journal

• Scientific reports

  Scientific reports 6 33548-, 2016-09-15

  Nature Publishing Group

Details

• CRID: 1050564288975040256
• NII Article ID: 120005855947
• HANDLE: 2115/63450
• ISSN: 20452322
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles