Dark-field X-ray ptychography: Towards high-resolution imaging of thick and unstained biological specimens

• Suzuki, Akihiro

• Shimomura, Kei

• Hirose, Makoto

• Burdet, Nicolas

• Takahashi, Yukio

抄録

The phase shift of light or electrons in objects is now necessary for probing weak-phase objects such as unstained biological specimens. Optical microscopy (OM) and transmission electron microscopy (TEM) have been used to observe weak-phase objects. However, conventional OM has low spatial resolution and TEM is limited to thin specimens. Here, we report on the development of dark-field X-ray ptychography, which combines X-ray ptychography and X-ray in-line holography, to observe weak-phase objects with a phase resolution better than 0.01 rad, a spatial resolution better than 15 nm, and a field of view larger than 5 mu m. We apply this method to the observation of both the outline and magnetosomes of the magnetotactic bacteria MO-1. Observation of thick samples with high resolution is expected to find broad applications in not only biology but also materials science.

収録刊行物

• Scientific reports

  Scientific reports 6 35060-, 2016-10-13

  Nature Publishing Group

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050001339021723008
• NII論文ID: 120005890051
• DOI: 10.1038/srep35060
• HANDLE: 2115/63743
• ISSN: 20452322
• Web Site: https://www.nature.com/articles/srep35060.pdf
• Web Site: https://www.nature.com/articles/srep35060
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN