MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin

• Kawashita, Masakazu

• Hayashi, Jumpei

• Kudo, Tada-aki

• Kanetaka, Hiroyasu

• Li, Zhixia

• Miyazaki, Toshiki

• Hashimoto, Masami

Abstract

Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.

Journal

• Journal of biomedical materials research. Part A

  Journal of biomedical materials research. Part A 102 (6), 1880-1886, 2013-07-24

  John Wiley & Sons, Inc.

Details

• CRID: 1050845763841023232
• NII Article ID: 120005890547
• NII Book ID: AA11881516
• ISSN: 15493296
• ISSN: 15524965
• HANDLE: 10228/5931
• Web Site: https://kyutech.repo.nii.ac.jp/records/4711
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles