Analysis on transglutaminase 1 and its substrates using specific substrate peptide in cultured keratinocytes

• Yamane, Miki

• Sugimura, Kayoko

• Kawasaki, Hiroko

• Tatsukawa, Hideki

• Hitomi, Kiyotaka

抄録

Transglutaminase (TGase) catalyzes protein cross-linking reactions essential for several biological processes. In differentiating keratinocytes, TG1 (keratinocyte-type) is crucial for the cross-linking of substrate proteins required for the complete formation of the cornified envelop, a proteinaceous supermolecule located in the outermost layer of the epidermis. TG1 expressions and its substrate were induced in cultured keratinocytes at differentiation-stage specific manner. In the cultured keratinocytes, we used the TG1-specific substrate peptide, which enables the specific detection of enzymatic activity to investigate its induction patterns. As a further application of the substrate peptide, several substrate candidates of TG1 that may be essential for cornified envelope formation were identified and characterized.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 478 (1), 343-348, 2016-09

  Elsevier

キーワード

• Transglutaminase
• Keratinocyte
• Epidermis

詳細情報

• CRID: 1050001338804884224
• NII論文ID: 120005893262
• HANDLE: 2237/25155
• ISSN: 0006291X
• Web Site: https://nagoya.repo.nii.ac.jp/records/22974
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles