PET imaging-guided chemogenetic silencing reveals a critical role of primate rostromedial caudate in reward evaluation.
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脳内に「やる気」のスイッチ、目で見て操作 : 霊長類の生体脳で人工受容体を画像化する技術を確立、高次脳機能研究の飛躍的な進展に期待. 京都大学プレスリリース. 2016-12-16.
The rostromedial caudate (rmCD) of primates is thought to contribute to reward value processing, but a causal relationship has not been established. Here we use an inhibitory DREADD (Designer Receptor Exclusively Activated by Designer Drug) to repeatedly and non-invasively inactivate rmCD of macaque monkeys. We inject an adeno-associated viral vector expressing the inhibitory DREADD, hM4Di, into the rmCD bilaterally. To visualize DREADD expression in vivo, we develop a non-invasive imaging method using positron emission tomography (PET). PET imaging provides information critical for successful chemogenetic silencing during experiments, in this case the location and level of hM4Di expression, and the relationship between agonist dose and hM4Di receptor occupancy. Here we demonstrate that inactivating bilateral rmCD through activation of hM4Di produces a significant and reproducible loss of sensitivity to reward value in monkeys. Thus, the rmCD is involved in making normal judgments about the value of reward.
- Nature communications
Nature communications (7), 2016-12-06