Expression of<i>N</i>-Acetylgalactosamine 4-Sulfate 6-<i>O</i>-Sulfotransferase Involved in Chondroitin Sulfate Synthesis Is Responsible for Pulmonary Metastasis

• Mizumoto, Shuji

• Watanabe, Moto

• Yamada, Shuhei

• Sugahara, Kazuyuki

書誌事項

タイトル別名

• Expression of N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase Involved in Chondroitin Sulfate Synthesis Is Responsible for Pulmonary Metastasis
• Expression of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase involved in chodroitin sulfate synthesis is responsible for pulmonary metastasis

抄録

Chondroitin sulfate (CS) containing E-disaccharide units, glucuronic acid-N-acetylgalactosamine(4, 6-O-disulfate), at surfaces of tumor cells plays a key role in tumor metastasis. However, the molecular mechanism of the metastasis involving the CS chaincontaining E-units is not fully understood. In this study, to clarify the role of E-units in the metastasis and to search for potential molecular targets for anticancer drugs, the isolation and characterization of Lewis lung carcinoma (LLC) cells stably downregulated by the knockdown for the gene encoding N-acetylgalactosamine 4-O-sulfate 6-O-sulfotransferase (GalNAc4S-6ST), which is responsible for the formation of E-units in CS chains, were performed. Knockdown of GalNAc4S-6ST in LLC cells resulted in a reduction in the proportion of E-units, in adhesiveness to extracellular matrix adhesion molecules and in proliferation in vitro. Furthermore, the stable downregulation of GalNAc4S-6ST expression in LLC cells markedly inhibited the colonization of the lungs by inoculated LLC cells and invasive capacity of LLC cells. ese results provide clear evidence that CS chain-containing E-units and/or GalNAc4S-6ST play a crucial role in pulmonary metastasis at least through the increased adhesion and the invasive capacity of LLC cells and also provides insights into future drug targets for anticancer treatment.

収録刊行物

• BioMed Research International

  BioMed Research International 2013 1-9, 2013

  Hindawi Publishing Corporation

キーワード

• General Immunology and Microbiology
• General Biochemistry, Genetics and Molecular Biology
• General Medicine

詳細情報

• CRID: 1050845763950800256
• NII論文ID: 120005906914
• DOI: 10.1155/2013/656319
• ISSN: 23146141
• ISSN: 23146133
• HANDLE: 2115/62915
• Web Site: http://downloads.hindawi.com/journals/bmri/2013/656319.pdf
• Web Site: http://downloads.hindawi.com/journals/bmri/2013/656319.xml
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN