Inhibition of cell polarity establishment in stomatal asymmetric cell division using the chemical compound bubblin

• 西村, いくこ

• 嶋田, 知生

• Kawase, Takashi

• Shirakawa, Makoto

• Imai, Yu

• Kawamoto, Yusuke

• Sugiyama, Hiroshi

• Nakagawa, Tsuyoshi

• Hara-Nishimura, Ikuko

• Shimada, Tomoo

書誌事項

タイトル別名

• The chemical compound bubblin induces stomatal mispatterning in Arabidopsis by disrupting the intrinsic polarity of stomatal lineage cells

抄録

Stem cell polarization is a crucial step in asymmetric cell division, which is a universal system for generating cellular diversity in multicellular organisms. Several conventional genetics studies have attempted to elucidate the mechanisms underlying cell polarization in plants, but it remains largely unknown. In plants, stomata, which are valves for gas exchange, are generated through several rounds of asymmetric divisions. In this study, we identified and characterized a chemical compound that affects stomatal stem cell polarity. Highthroughput screening for bioactive molecules identified a pyridinethiazole derivative, named bubblin, which induced stomatal clustering in Arabidopsis epidermis. Bubblin perturbed stomatal asymmetric division, resulting in the generation of two identical daughter cells. Both cells continued to express the stomatal fate determinant SPEECHLESS, and then differentiated into mispatterned stomata. Bubblin-treated cells had a defect in the polarized localization of BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), which is required for asymmetric cell fate determination. Our results suggest that bubblin induces stomatal lineage cells to divide without BASL-dependent pre-mitotic establishment of polarity. Bubblin is a potentially valuable tool for investigating cell polarity establishment in stomatal asymmetric division.

収録刊行物

• Development

  Development 144 (3), 499-506, 2017-02-01

  Company of Biologists Ltd

キーワード

• Asymmetric division
• Cell polarity
• Stomata
• Chemical biology
• Arabidopsis thaliana

詳細情報

• CRID: 1050564285794512000
• NII論文ID: 120005980855
• ISSN: 09501991
• ISSN: 14779129
• DOI: 10.1242/dev.145458
• HANDLE: 2433/218463
• Web Site: https://syndication.highwire.org/content/doi/10.1242/dev.145458
• Web Site: http://journals.biologists.com/dev/article-pdf/144/3/499/1850346/dev145458.pdf
• Web Site: http://journals.biologists.com/dev/article-pdf/doi/10.1242/dev.145458/2052964/dev_145458v1.pdf
• 本文言語コード: en
• 資料種別: journal article
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