Beetroot betalain inhibits peroxynitrite-mediated tyrosine nitration and DNA strand cleavage

• Sakihama, Yasuko

• Maeda, Makiko

• Hashimoto, Makoto

• Tahara, Satoshi

• Hashidoko, Yasuyuki

Abstract

Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO-) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO-, and the absorbance decreases were suppressed in the presence of glutathione, a ONOO- scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red beetroot betacyanins, treated with ONOO- monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO- concentration. Betanin inhibited the ONOO- (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC50 value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05-1.0 μM) also inhibited ONOO- (0.5 mM)-dependent DNA strand cleavage in a concentration-dependent manner. These results suggest that betalains can protect cells from nitrosative stress in addition to protecting them from oxidative stresses.

Journal

• Free Radical Research

  Free Radical Research 46 (1), 93-99, 2012-01

  Taylor & Francis

Keywords

• betanin
• nitrosative stress
• plant pigment
• reactive nitrogen species
• reactive oxygen species

Details

• CRID: 1050564288976233856
• NII Article ID: 120005981376
• DOI: 10.3109/10715762.2011.641157
• ISSN: 10292470
• ISSN: 10715762
• HANDLE: 2115/64661
• Web Site: http://www.tandfonline.com/doi/pdf/10.3109/10715762.2011.641157
• Text Lang: en
• Article Type: journal article
• Data Source:
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  • Crossref
  • CiNii Articles
  • KAKEN