SHISA6 Confers Resistance to Differentiation-Promoting Wnt/β-Catenin Signaling in Mouse Spermatogenic Stem Cells

• Tokue, Moe

• Ikami, Kanako

• Mizuno, Seiya

• Takagi, Chiyo

• Miyagi, Asuka

• Takada, Ritsuko

• Noda, Chiyo

• Kitadate, Yu

• Hara, Kenshiro

• Mizuguchi, Hiroko

• Sato, Takuya

• Taketo, Makoto Mark

• Sugiyama, Fumihiro

• Ogawa, Takehiko

• Kobayashi, Satoru

• Ueno, Naoto

• Takahashi, Satoru

• Takada, Shinji

• Yoshida, Shosei

Bibliographic Information

Other Title

• SHISA6 confers resistance to differentiation-promoting Wnt/beta-catenin signaling in mouse spermatogenic stem cells

Abstract

In the seminiferous tubules of mouse testes, a population of glial cell line-derived neurotrophic factor family receptor alpha 1 (GFRα1)-positive spermatogonia harbors the stem cell functionality and supports continual spermatogenesis, likely independent of asymmetric division or definitive niche control. Here, we show that activation of Wnt/β-catenin signaling promotes spermatogonial differentiation and reduces the GFRα1+ cell pool. We further discovered that SHISA6 is a cell-autonomous Wnt inhibitor that is expressed in a restricted subset of GFRα1+ cells and confers resistance to the Wnt/β-catenin signaling. Shisa6+ cells appear to show stem cell-related characteristics, conjectured from the morphology and long-term fates of T (Brachyury)+ cells that are found largely overlapped with Shisa6+ cells. This study proposes a generic mechanism of stem cell regulation in a facultative (or open) niche environment, with which different levels of a cell-autonomous inhibitor (SHISA6, in this case) generates heterogeneous resistance to widely distributed differentiation-promoting extracellular signaling, such as WNTs.

Journal

• Stem Cell Reports

  Stem Cell Reports 8 561-575, 2017-02-09

  Elsevier B.V.

Keywords

• Cell Biology
• Developmental Biology
• Genetics
• Biochemistry

Details

• CRID: 1050001335841191296
• NII Article ID: 120005997826
• NII Article ID: 120007129230
• ISSN: 22136711
• DOI: 10.1016/j.stemcr.2017.01.006
• HANDLE: 2433/218789
• HANDLE: 2241/00145986
• Web Site: https://tsukuba.repo.nii.ac.jp/records/41038
• Web Site: https://api.elsevier.com/content/article/PII:S2213671117300218?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S2213671117300218?httpAccept=text/plain
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN