Dissecting in vivo steady-state dynamics of karyopherin-dependent nuclear transport

• 粂田, 昌宏

  京都大学大学院生命科学研究科

• 吉村, 成弘

• Otsuka, Shotaro

• Kumeta, Masahiro

• Yoshimura, Shige H.

抄録

Karyopherin-dependent molecular transport through the nuclear pore complex is maintained by constant recycling pathways of karyopherins coupled with the Ran-dependent cargo catch-and-release mechanism. Although many studies have revealed the bidirectional dynamics of karyopherins, the entire kinetics of the steady-state dynamics of karyopherin and cargo is still not fully understood. In this study, we used fluorescence recovery after photobleaching and fluorescence loss in photobleaching on live cells to provide convincing in vivo proof that karyopherin-mediated nucleocytoplasmic transport of cargoes is bidirectional. Continuous photobleaching of the cytoplasm of live cells expressing NLS cargoes led to progressive decrease of nuclear fluorescence signals. In addition, experimentally obtained kinetic parameters of karyopherin complexes were used to establish a kinetic model to explain the entire cargo import and export transport cycles facilitated by importin β. The results strongly indicate that constant shuttling of karyopherins, either free or bound to cargo, ensures proper balancing of nucleocytoplasmic distribution of cargoes and establishes effective regulation of cargo dynamics by RanGTP.

収録刊行物

• Molecular Biology of the Cell

  Molecular Biology of the Cell 27 (1), 167-176, 2016-01-01

  American Society for Cell Biology

キーワード

• Cell Biology
• Molecular Biology

詳細情報

• CRID: 1050845760771364224
• NII論文ID: 120005997938
• ISSN: 19394586
• HANDLE: 2433/218972
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles