The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane

• 加藤, 洋平

  Graduate School of Pharmaceutical Sciences, Kyoto University

• 中山, 和久

• 申, 惠媛

• Tanaka, Gaku

• Katoh, Yohei

• Nakayama, Kazuhisa

• Takatsu, Hiroyuki

• Shin, Hye Won

抄録

Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane.

収録刊行物

• Molecular Biology of the Cell

  Molecular Biology of the Cell 27 (24), 3883-3893, 2016-12-01

  American Society for Cell Biology

詳細情報

• CRID: 1050564285794654976
• NII論文ID: 120005997942
• ISSN: 19394586
• HANDLE: 2433/218976
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles