High-speed atomic force microscopy for observing protein molecules in dynamic action

• Ando, Toshio

抄録

Directly observing protein molecules in dynamic action at high spatiotemporal resolution has long been a holy grail for biological science. To materialize this long quested dream, I have been developing high-speed atomic force microscopy (HS-AFM) since 1993. Tremendous strides were recently accomplished in its high-speed and low-invasive performances. Consequently, various dynamic molecular actions, including bipedal walking of myosin V and rotary propagation of structural changes in F1-ATPase, were successfully captured on video. The visualized dynamic images not only provided irrefutable evidence for speculated actions of the protein molecules but also brought new discoveries inaccessible with other approaches, thus giving great mechanistic insights into how the molecules function. HS-AFM is now transforming "static" structural biology into dynamic structural bioscience. © 2017 SPIE.

収録刊行物

• Proceedings of SPIE - The International Society for Optical Engineering

  Proceedings of SPIE - The International Society for Optical Engineering 10328 103281R-, 2017-01-01

  SPIE

キーワード

• High-speed AFM
• proteins
• dynamic processes
• high frame rate
• atomic force microscopy

詳細情報

• CRID: 1050001335942459264
• NII論文ID: 120006306248
• NII書誌ID: AA10619755
• ISSN: 0277786X
• Web Site: http://hdl.handle.net/2297/47884
• 本文言語コード: en
• 資料種別: journal article
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