R26-WntVis reporter mice showing graded response to Wnt signal levels

• Takemoto, Tatsuya

• Abe, Takaya

• Kiyonari, Hiroshi

• Nakao, Kazuki

• Furuta, Yasuhide

• Suzuki, Hitomi

• Takada, Shinji

• Fujimori, Toshihiko

• Kondoh, Hisato

Abstract

The canonical Wnt signaling pathway plays a major role in the regulation of embryogenesis and organogenesis, where signal strength-dependent cellular responses are of particular importance. To assess Wnt signal levels in individual cells, and to circumvent the integration site-dependent bias shown in previous Wnt reporter lines, we constructed a new Wnt signal reporter mouse line R26-WntVis. Heptameric TCF/LEF1 binding sequences were combined with a viral minimal promoter to confer a graded response to the reporter depending on Wnt signal strengths. The histone H2B-EGFP fusion protein was chosen as the fluorescent reporter to facilitate single-cell resolution analyses. This WntVis reporter gene was then inserted into the ROSA26 locus in an orientation opposite to that of the endogenous gene. The R26-WntVis allele was introduced into Wnt3a−/− and Wnt3avt/− mutant mouse embryos and compared with wild-type embryos to assess its performance. The R26-WntVis reporter was activated in known Wnt-dependent tissues and responded in a graded fashion to signal intensity. This analysis also indicated that the major Wnt activity early in embryogenesis switched from Wnt3 to Wnt3a around E7.5. The R26-WntVis mouse line will be widely useful for the study of Wnt signal-dependent processes.

Journal

• Genes to Cells

  Genes to Cells 21 (6), 661-669, 2016-03-31

  Wiley

Keywords

• Cell Biology
• Genetics

Details

• CRID: 1050001337465057792
• NII Article ID: 120006306641
• NII Book ID: AA11623816
• ISSN: 13652443
• ISSN: 13569597
• DOI: 10.1111/gtc.12364
• Web Site: https://repo.lib.tokushima-u.ac.jp/110152
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fgtc.12364
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/gtc.12364
• Web Site: https://onlinelibrary.wiley.com/doi/full-xml/10.1111/gtc.12364
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN