Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles

• Inagaki, Eri

• Sakai, Mizuki

• Hirano, Minato

• Muto, Memi

• Kobayashi, Shintaro

• Kariwa, Hiroaki

• Yoshii, Kentaro

抄録

Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. A wide range of animal species could be infected with TBEV in endemic areas. A serological survey of wild animals is effective in identifying TBEV-endemic areas. Safe, simple, and reliable TBEV serodiagnostic tools are needed to test animals. In this study, ELISA was developed to detect anti-TBEV specific antibodies in multi-species of animals, using recombinant subviral particles (SPs) with an affinity tag and protein A/G. A Strep-tag was fused at the N terminus of the E protein of the plasmid coding TBEV prME. The E proteins with Strep-tag were secreted as SPs, of which Strep-tag was exposed on the surface. The tagged E proteins were associated with prM. The SPs with Strep-tag were applied as the antigen of ELISA, and TBEV-specific antibodies were detected by the protein A/G. Compared to neutralization test results, the ELISA showed 96.8% sensitivity and 97.7% specificity in rodents and 95.1% sensitivity and 96.0% specificity in humans, without cross-reactivity with antibodies to Japanese encephalitis virus. These results indicate that our ELISA would be useful to detect TBE-specific antibodies in a wide range of animal species. (C) 2016 Elsevier GmbH. All rights reserved.

収録刊行物

• Ticks and Tick-Borne Diseases

  Ticks and Tick-Borne Diseases 7 (5), 723-729, 2016-07

  Elsevier

キーワード

• Tick-borne encephalitis virus
• Flavivirus
• Subviral particles
• ELISA

詳細情報

• CRID: 1050001202675106176
• NII論文ID: 120006318731
• ISSN: 18779603
• ISSN: 1877959X
• HANDLE: 2115/66556
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles